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Updated: Aug 13, 2026
15:42
Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
Published on: March 6, 2009
Human memory T cells express intercellular adhesion molecule-1 which can be increased by interleukin 2 and
1Macrophage Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London.
European Journal of Immunology
|February 1, 1990
Summary
Memory T cells express intercellular adhesion molecule-1 (ICAM-1), enabling rapid recall antigen responses. ICAM-1 expression on T cells is upregulated by cytokines and blocking it inhibits antigen response, indicating its functional role.
Area of Science:
- Immunology
- Cell Biology
Background:
- Intercellular adhesion molecule-1 (ICAM-1) is expressed on T cells.
- Memory T cells possess distinct phenotypic and functional characteristics compared to naive T cells.
Purpose of the Study:
- To investigate the expression and function of ICAM-1 on peripheral blood T cells.
- To determine the role of ICAM-1 in T cell activation and response to recall antigens.
Main Methods:
- Flow cytometry to detect ICAM-1 expression on T cells.
- Functional assays to assess T cell response to recall antigens.
- Cytokine stimulation assays to evaluate ICAM-1 upregulation.
Main Results:
- Approximately 40% of resting peripheral blood T cells express ICAM-1.
- ICAM-1+ T cells exhibit memory cell markers and respond rapidly to recall antigens.
- Interleukin-2 and interferon-gamma increase ICAM-1 expression on T cells.
- Anti-ICAM-1 antibody treatment inhibits T cell response to recall antigens.
Conclusions:
- ICAM-1 is a functional marker on memory T cells involved in recall antigen responses.
- Cytokines regulate ICAM-1 expression, suggesting a role in T cell activation.
- ICAM-1 plays a significant role in the functional capabilities of memory T cells.
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