Related Experiment Video
Updated: Aug 6, 2026

Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction
Published on: January 12, 2016
Direct association between caspase 3 and alpha5beta1 integrin and its role during anoikis of rat fibroblasts
1Centre for Cellular and Molecular Biology, Cell Biology Department, Habsiguda, Uppal Road, Hyderabad, Andhra Pradesh 500 007, India.
Abstract:
We have demonstrated a direct association between alpha5beta1 integrin and caspase 3, both pro- and mature enzyme, in various sub-cellular compartments of rat fibroblasts undergoing anoikis. Integrin associated caspase 3 showed high activity in the plasma membranes, whereas in the cytosol and microsomal fraction it showed little or no activity. Our results suggest a possible role for recycled un-ligated alpha5beta1 integrin molecules between cytosol and plasma membrane, in regulation of caspase-3 activity and induction of cell death in adhesion-deprived cells.
Related Concept Videos
Intracellular Signaling Affects Focal Adhesions
Some...
Caspases
The Extrinsic Apoptotic Pathway
The Intrinsic Apoptotic Pathway
Activation of Integrins
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
Integrins
Some ECM proteins assemble into a basement membrane to which the remaining components adhere. Proteoglycans typically form the bulk of the ECM while fibrous proteins, like collagen,...

