Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Visualizing plant development and gene expression in three dimensions using optical projection tomography.

Karen Lee1, Jerome Avondo, Harris Morrison

  • 1Department of Cell and Developmental Biology, John Ines Centre, Norwich Research Park, Norwich, NR4 7UH United Kingdom.

The Plant Cell
|August 15, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Implementation Evaluation of a Multicomponent Intervention to Address the Infectious Disease and Overdose Syndemic Among People Who Use Drugs in Rural Settings.

Journal of public health management and practice : JPHMP·2026
Same author

Microbial characterization of oral microbiome in patients with open-angle glaucoma.

Scientific reports·2026
Same author

ASO Visual Abstract: Comparing Resident Team Performance in Complex Surgical Oncology: A Single-Institution Cohort Study.

Annals of surgical oncology·2026
Same author

A Scalability Assessment of an Organizational Health Literacy Initiative to Support Health Professionals in Developing Quality Consumer Information.

Journal of health communication·2026
Same author

Patient Outcome Reports for Senior Residents: An Opportunity to Foster Reflection and Identify Outliers for Intervention.

Journal of surgical education·2026
Same author

Synthetic pectin-cellulose nanofiber capsule recapitulates the mechanical properties of a regenerating plant cell wall.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

The Plant RABC1 GTPase Coordinates with Exocyst Component SEC5A in Regulating ER-phagy under Endoplasmic Reticulum Stress.

The Plant cell·2026
Same journal

Graft transmissible resistance to Alternaria alternata is mediated by rootstock to scion JA transport activating raffinose synthesis.

The Plant cell·2026
Same journal

What fresh cell is this? Building a single-cell atlas of developing grass leaves in Brachypodium distachyon.

The Plant cell·2026
Same journal

The STA1-DOT2 interaction promotes nuclear speckle formation and splicing robustness in growth and heat stress responses.

The Plant cell·2026
Same journal

GIGANTEA shapes diurnal seedling growth by sequestering SMAX1 and SMXL2.

The Plant cell·2026
Same journal

Vascular-specific genome editing enhances low-phosphate tolerance in rice.

The Plant cell·2026
See all related articles

Optical projection tomography (OPT) provides detailed 3D plant imaging for studying growth and gene activity across various plant parts and scales. This method enables quantitative analysis of plant morphology and gene expression, advancing developmental biology research.

Area of Science:

  • Plant Biology
  • Developmental Biology
  • Imaging Technology

Background:

  • Understanding plant growth requires 3D data on morphology and gene activity.
  • Current methods may lack the resolution or scope needed for comprehensive analysis.

Purpose of the Study:

  • To explore optical projection tomography (OPT) for capturing 3D plant data.
  • To demonstrate OPT's utility across diverse plant materials and scales.
  • To enable quantitative analysis of plant development and gene expression.

Main Methods:

  • Application of optical projection tomography (OPT) to various plant specimens (seedlings, leaves, flowers, roots, seeds, embryos, meristems).
  • High-resolution imaging to visualize cellular structures within the whole plant context.

Related Experiment Videos

  • Live 3D imaging for semitransparent structures like roots.
  • Visualization of 3D gene expression domains using marker genes or whole-mount in situ hybridization.
  • Development of tools and software for interactive 3D data quantification and visualization.

    • Main Results:

    • OPT successfully imaged diverse plant materials at multiple scales, from whole seedlings to individual cells.
    • Live 3D imaging was feasible for transparent tissues.
    • 3D gene expression patterns were visualized effectively.
    • Tools for interactive data analysis and visualization were developed.

    Conclusions:

    • Optical projection tomography is a versatile and powerful technique for quantitative 3D analysis of plant morphology and gene expression.
    • OPT facilitates a deeper understanding of plant growth and development mechanisms.
    • The developed methods and tools support advanced research in plant science.