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Multiparallel chiral method development screening using an 8-channel microfluidic HPLC system.

Peter Sajonz1, Xiaoyi Gong, William R Leonard

  • 1Separation & Analysis Technologies, Process Research, Merck & Co. Inc., Rahway, New Jersey 07065, USA. peter_sajonz@merck.com

Chirality
|August 15, 2006
PubMed
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Multiparallel screening using the Eksigent Express 800 system enables rapid development of fast normal phase chiral separations. This microfluidic HPLC approach optimizes methods in under an hour, significantly faster than traditional methods.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Traditional chiral method development is time-consuming, often requiring overnight results.
  • Automated sequential approaches present limitations in speed and efficiency for chiral separations.

Purpose of the Study:

  • To investigate the efficacy of the Eksigent Express 800 8-channel microfluidic HPLC system for multiparallel screening.
  • To develop fast normal phase chiral separation methods with near real-time optimization.
  • To compare the performance of microfluidic HPLC with conventional HPLC systems.

Main Methods:

  • Utilized the Eksigent Express 800 8-channel microfluidic HPLC system.
  • Screened four column types (300 µm i.d.) with two mobile phases using a universal gradient approach.
  • Analyzed standard test racemates to develop optimized separation methods.

Related Experiment Videos

Main Results:

  • Achieved optimized chiral separation methods in the 1- to 2-minute range for most racemates.
  • Demonstrated near real-time method development, with optimized methods often obtained in less than 1 hour.
  • Observed that parallel method optimization yielded surprising and valuable outcomes.
  • Showed favorable comparison with results from conventional HPLC systems using larger columns (4.6 mm i.d.).
  • Confirmed successful translation of isocratic methods from microbore to larger column formats.

Conclusions:

  • The microfluidic HPLC system facilitates rapid and efficient multiparallel screening for chiral separations.
  • Near real-time method development is achievable, significantly reducing the time required for optimization.
  • The multiparallel approach offers advantages over sequential methods, revealing unexpected optimization pathways.
  • Microfluidic systems provide a viable and efficient alternative to conventional HPLC for fast chiral method development and analysis.