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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
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High-throughput and high-resolution flow cytometry in molded microfluidic devices.

Claire Simonnet1, Alex Groisman

  • 1Department of Physics, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, USA.

Analytical Chemistry
|August 16, 2006
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Summary

Researchers developed two microfluidic flow cytometers using poly(dimethylsiloxane) for high-throughput particle analysis and high-resolution cell imaging. These devices offer accurate fluorescence detection and detailed cellular analysis in continuous flow.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Flow cytometry is a crucial technique for cell analysis.
  • Existing flow cytometers can be expensive and complex.
  • Microfluidic devices offer potential for miniaturized and cost-effective analytical systems.

Purpose of the Study:

  • To design, fabricate, and operate two novel microfluidic flow cytometers.
  • To achieve high-throughput particle analysis and high-resolution cell imaging.
  • To explore applications in inexpensive stand-alone cytometers and integrated microanalysis systems.

Main Methods:

  • Fabrication of microfluidic devices using poly(dimethylsiloxane) (PDMS).
  • Hydrodynamic focusing for precise particle/cell stream control (transverse and lateral).
  • Integration with fluorescence detection and microscopy for analysis.

Main Results:

  • First device: Achieved fluorescence detection accuracy comparable to commercial systems, with throughput up to 17,000 particles/s.
  • Second device: Focused particles into a submicrometer layer for high-resolution imaging.
  • High-resolution device enabled imaging comparable to still micrographs with accurate balancing of particle motion.

Conclusions:

  • Microfluidic flow cytometers offer a viable alternative to conventional systems.
  • The high-throughput device is suitable for inexpensive cytometers or integrated systems.
  • The high-resolution device facilitates detailed cellular morphology and fluorescence distribution analysis in continuous flow.