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Analysis of variation in batches of armadillo-derived Mycobacterium leprae by immunoblotting
M A Ibrahim1, F I Lamb, M J Colston
1Laboratory for Leprosy and Mycobacterial Research, National Institute for Medical Research, Mill Hill, London, U.K.
Abstract:
Several batches of cell-free extracts of armadillo-derived Mycobacterium leprae were analyzed by SDS-PAGE and by immunoblotting with monoclonal antibodies. The presence or absence of protease inhibitors had a profound effect on the protein antigens, particularly the 65-kDa antigen. In the absence of protease inhibitors, there were both quantitative and qualitative differences between the different batches of M. leprae extracts.