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An amperometric uric acid biosensor based on modified Ir-C electrode.

Yung-Chien Luo¹, Jing-Shan Do, Chung-Chiun Liu

¹Center of Nanoscience, Nanotechnology, Department of Chemical Engineering, Tunghai University, Taichung, Taiwan 40704, ROC.

Biosensors & Bioelectronics

|August 16, 2006

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A novel uricase/Ir-C biosensor accurately detects uric acid (UA) levels. This amperometric sensor offers high selectivity and a low detection limit, aiding in diagnosing conditions like gout and kidney failure.

Area of Science:

Electrochemistry
Biosensors
Biomedical Engineering

Background:

Uric acid (UA) levels are critical indicators for gout, hyperuricemia, Lesch-Nyan syndrome, and kidney failure.
Accurate UA determination is essential for clinical diagnosis and monitoring.

Purpose of the Study:

To develop and characterize a novel amperometric uric acid biosensor.
To utilize an iridium-modified carbon (Ir-C) electrode with immobilized uricase for enhanced UA detection.

Main Methods:

Fabrication of an Ir-C working electrode using thick film screen printing.
Immobilization of uricase enzyme onto the Ir-C electrode.
Amperometric detection of UA using chronoamperometry (CA) at a potential of 0.25 V (vs. Ag/AgCl).

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Main Results:

The developed biosensor demonstrated high selectivity for UA due to reduced H(2)O(2) oxidation potential on the Ir-C electrode.
Minimal interference was observed from ascorbic acid (AA) and other electroactive species.
Sensitivity of 16.60 microAmM(-1) was achieved for UA detection in the 0.1-0.8 mM range.
A low detection limit of 0.01 mM (S/N=6.18) was obtained under optimal conditions (pH 7 PBS, 37°C).

Conclusions:

The uricase/Ir-C biosensor provides a sensitive and selective platform for amperometric UA determination.
This biosensor shows promise for clinical applications in diagnosing UA-related disorders.
Optimization of pH, temperature, and enzyme loading further enhances biosensor performance.