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Published on: July 24, 2021
Hyperinduction of nitrilases in filamentous fungi
Ondrej Kaplan1, Vojtech Vejvoda, Andrea Charvátová-Pisvejcová
1Laboratory of Biotransformation, Institute of Microbiology, Academy of Sciences of Czech Republic, Vídenská 1083, 142 20 Prague 4, Czech Republic.
Abstract:
2-Cyanopyridine proved to act as a powerful nitrilase inducer in Aspergillus niger K10, Fusarium solani O1, Fusarium oxysporum CCF 1414, Fusarium oxysporum CCF 483 and Penicillium multicolor CCF 2244. Valeronitrile also enhanced the nitrilase activity in most of the strains. The highest nitrilase activities were produced by fungi cultivated in a Czapek-Dox medium with both 2-cyanopyridine and valeronitrile. The specific nitrilase activities of these cultures were two to three orders of magnitude higher than those of cultures grown on other nitriles such as 3-cyanopyridine or 4-cyanopyridine.
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