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Laboratory identification and sensitivity testing of yeast isolates.

¹National Institute of Public Health, Oslo, Norway.

Acta Odontologica Scandinavica

|February 1, 1990

Summary

Accurate yeast identification relies on morphology and biochemistry, with germ tube and carbohydrate assimilation tests being key. Antifungal susceptibility testing lacks standardization, recommending preliminary screening for specific drugs in smaller labs.

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Area of Science:

Medical Mycology
Clinical Microbiology

Background:

Accurate identification of yeast pathogens is crucial for effective treatment.
Standardized methods for antifungal susceptibility testing are lacking, leading to variable results.

Purpose of the Study:

To outline reliable methods for yeast identification.
To discuss current approaches and limitations in antifungal susceptibility testing.

Main Methods:

Yeast identification utilizes morphologic features and biochemical characteristics.
Key identification tests include the germ tube test for Candida albicans and carbohydrate assimilation tests for other species.
Commercially available identification kits are generally satisfactory.

Main Results:

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The germ tube test is a rapid method for identifying Candida albicans.
Carbohydrate assimilation tests are essential for identifying other yeast species.
Antifungal susceptibility testing methods are not standardized, yielding inconsistent results.

Conclusions:

A combination of morphologic and biochemical tests ensures accurate yeast identification.
Smaller laboratories should perform preliminary antifungal susceptibility screening for flucytosine and amphotericin B.
Complex or critical cases warrant referral to specialized laboratories for comprehensive antifungal susceptibility testing.