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Related Experiment Videos

T-cell receptor expression in the T-cell malignancies.

N Mori1, K Oka, Y Yoda

  • 1Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki, Japan.

American Journal of Clinical Pathology
|April 1, 1990
PubMed
Summary

T-cell receptor (TCR) beta subunit expression precedes CD1 expression in T-cell neoplasms. TCR beta is found in the cytoplasm, while TCR alpha beta is on the cell surface.

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Area of Science:

  • Immunology
  • Oncology
  • Cell Biology

Background:

  • T-cell neoplasms are a diverse group of cancers.
  • Understanding T-cell receptor (TCR) expression is crucial for diagnosis and classification.
  • Previous studies have provided insights into TCR subunit expression during T-cell differentiation.

Purpose of the Study:

  • To investigate the expression patterns of TCR beta and alpha beta subunits in various T-cell neoplasms.
  • To determine the relationship between TCR beta expression and CD1 expression in immature T-cells.
  • To evaluate the utility of specific monoclonal antibodies (beta F1, delta TCS1, WT31) for detecting TCR subunits in T-cell malignancies.

Main Methods:

  • Immunohistochemical staining of 28 T-cell neoplasms and 2 reactive lymph nodes using beta F1, delta TCS1, and WT31 antibodies.

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  • Analysis of TCR subunit localization (cytoplasmic vs. surface membrane) using immuno-electron microscopy.
  • Correlation of TCR expression with CD1 (OKT6) expression.

    • Main Results:

    • TCR beta subunit (detected by beta F1) was expressed in T-cell acute lymphoblastic leukemia (T-ALL), T-lymphoblastic lymphoma (T-LL), and peripheral T-cell lymphomas.
    • No reactivity was observed for the TCR delta subunit (delta TCS1).
    • TCR alpha beta subunit (detected by WT31) showed reactivity on the surface membrane in some T-LL and peripheral T-cell lymphomas.
    • TCR beta expression was found to occur earlier than or concurrently with CD1 expression in immature T-cells.
    • TCR beta was localized predominantly in the cytoplasm, whereas WT31 reactivity was on the cell surface.

    Conclusions:

    • TCR beta subunit expression is an early event in T-cell differentiation, preceding or coinciding with CD1 expression.
    • TCR beta is primarily found in the cytoplasm of T-cells, while TCR alpha beta is a surface marker.
    • The WT31 antibody may have limited utility for detecting TCR alpha beta on T-cell neoplasms in frozen sections.