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Determination of poly(ADP-ribose) chain length distribution on polyacrylamide gels by silver staining
1Department of Biology, Technion-Israel Institute of Technology, Haifa.
Analytical Biochemistry
|January 1, 1990
Abstract:
Silver staining and polyacrylamide gel electrophoresis were used to visualize chain length distribution of poly(ADP-ribose) enzymatically synthesized from NAD by rat liver nuclei. The method described has the advantage that synthesis does not require radioactive-labeled NAD, and microgram quantities (greater than 5 micrograms) of poly(ADP-ribose) can be resolved and visualized as discrete bands according to chain lengths which range from 8 to 60 residues. This method can be applied to estimate size distribution of poly(ADP-ribose) chains in cells or tissues.