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Updated: Aug 6, 2026
Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers
Published on: October 21, 2018
[A study on the expression of HSP70 in endothelial cells pretreated with ethanol and fluid shear stress]
Hong-tao Yan1, Yi Zhang, Ga Liao
1Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China.
Objective:
To detect whether ethanol can affect the expression of HSP70 in endothelial cells under fluid shear stress.
Methods:
Ethanol at different concentrations was added to the culture medium of endothelial cells, EA. Hy926, which was treated statically or under 1Pa fluid shear stress. After the incubation of 1 h, 2 h, 4 h and 6 h, the expression of HSP70 was detected by immunohistochemical method(SP).
Results:
In the control group, the expression of HSP70 was negative under static state, while it was positive under 1Pa fluid shear stress lasting 4 h even without ethanol. No statistic difference was found between the 50 mg/dL ethanol group and the control group with the same treatment time of fluid shear stress. HSP70 expression was found under static state with 150 mg/dL ethanol after 4 h or 300 mg/dL ethanol after 2 h respectively. The expression increased greatly under 1Pa fluid shear stress in the same ethanol concentrations.
Conclusion:
Medium to high ethanol concentration in coordination with fluid shear stress can strongly stimulates the expression of HSP70 by a kinetic mechanism of time-dependent.
