[Proliferation and undifferentiation of spermatogonial stem cells in vitro]

To investigate the fluences on the proliferation and undifferentiation of spermatogonial stem cells in vitro.

Mouse bone marrow stromal cells were subjected to primary culture; when the cells grew up and extended around, they were treated with mitomycin C. And then, to the bone marrow stromal cells were added spermatogonial stem cells for co-culture. The ambient temperature was 37 degrees C.

The spermatogonial stem cells increased in number obviously when cultured for 2-4 days, they could be single, paired or clustered, while the differentiated cells began to die; when cultured for 4-8 days, the cells proliferated not in a distinct manner, they seemed to be stable; but at 10 days, the cells began to grow, especially when cultured for 15 days, the cells had the characteristic structure of the spermatogonial stem cell; when cultured continuously for 25 days, the cells had the same morphology and very clear intercellular bridges.

Spermatogonial stem cells can proliferate and continue to be undifferentiated on the bone marrow stromal cells when the ambient temperature is 37 degrees C.