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Generation of Recombinant Arenavirus for Vaccine Development in FDA-Approved Vero Cells
Published on: August 1, 2013
Early steps in avian reovirus morphogenesis
J Benavente1, J Martínez-Costas
1Departamento de Bioquímica y Biología Molecular, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Current Topics in Microbiology and Immunology
|August 17, 2006
Summary
Avian reoviruses cause significant poultry losses. This study models avian reovirus gene expression and morphogenesis, detailing how the muNS protein forms viral factories for replication and assembly.
Area of Science:
- Virology
- Molecular Biology
- Poultry Pathology
Background:
- Avian reoviruses are significant poultry pathogens causing economic losses.
- Viral entry involves receptor-mediated endocytosis and endosomal acidification for uncoating.
- Replication occurs in cytoplasmic inclusions called viral factories, formed by the muNS protein.
Purpose of the Study:
- To present a model for avian reovirus gene expression.
- To elucidate the process of avian reovirus morphogenesis.
- To understand the role of the muNS protein in viral factory formation and protein recruitment.
Main Methods:
- Analysis of avian reovirus gene expression.
- Observation of viral morphogenesis within infected cells.
- Comparison with data from other Reoviridae family members.
Main Results:
- Avian reoviruses replicate in non-microtubule-associated viral factories formed by muNS.
- MuNS protein mediates specific recruitment of viral proteins into factories.
- Core assembly and outer-capsid coating occur within a defined timeframe in viral factories.
Conclusions:
- A model for avian reovirus gene expression and morphogenesis is proposed.
- Viral factories are crucial sites for avian reovirus replication and assembly.
- The muNS protein plays a central role in organizing viral factories and controlling protein incorporation.
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