A validated method for halquinol extraction from chicken liver matrix for residual HPLC studies

Area of Science:

• Food safety analysis
• Veterinary drug residue detection
• Analytical chemistry

Background:

• Veterinary drug and pesticide residues in food pose risks to consumers.
• Accurate methods for residue determination are crucial for food safety assessment.
• Halquinol is a veterinary drug requiring specific analytical methods.

Purpose of the Study:

• To establish and validate a method for extracting and determining Halquinol in chicken liver.
• To ensure accurate quantification of Halquinol residues in food matrices.
• To contribute to the safety assessment of veterinary drug usage in poultry.

Main Methods:

• High-performance liquid chromatography (HPLC) with a C18 reversed-phase column and MWD-UV detector was used for determination.
• Chicken liver samples were homogenized, extracted with ethyl acetate, and purified using solid-phase extraction.
• Method validation included establishing limits of quantification and detection, and assessing recovery rates.

Main Results:

• The HPLC method was successfully established and validated for Halquinol determination.
• The limit of quantification was 0.1 microg/mL, and the detection limit was 0.01 microg/mL.
• Mean recoveries of Halquinol in chicken liver samples ranged from 57% to 91%.

Conclusions:

• A reliable method for Halquinol extraction and determination in chicken liver has been developed.
• This method supports the monitoring of veterinary drug residues in poultry products.
• The validated method aids in ensuring food safety and protecting consumer health.