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Updated: Aug 6, 2026

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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
[Vaginal swab in pediatric age]
S Beolchi1, C Mastromatteo, M Baietti
1U.O. Ostetricia e Ginecologia, Azienda ICP, Ospedale dei Bambini V. Buzzi, Milano, Italia.
Summary
The urethral cotton swab is the most successful tool for vaginal secretion sampling in young patients with suspected vulvovaginitis. This method demonstrated the highest patient compliance and lowest discontinuation rates in a pediatric study.
Area of Science:
- Pediatric Gynecology
- Clinical Diagnostics
Background:
- Vulvovaginitis is common in young patients.
- Accurate diagnosis relies on proper vaginal secretion sampling.
Purpose of the Study:
- To compare the efficacy of different sampling tools for vaginal secretions in pediatric patients.
- To assess patient tolerance and compliance with various sampling instruments.
Main Methods:
- A randomized prospective study involving 100 children (4 months-8 years) with suspected vulvovaginitis.
- Four instruments were tested: classical cotton swab, urethral cotton swab, balloon shape cotton swab, and cyto-brush.
- Sampling success, patient compliance, and discontinuation rates due to intolerance were evaluated for each tool.
Main Results:
- The urethral cotton swab had the highest sampling success rate (96%), with only 1 of 25 patients discontinuing the procedure.
- The classical cotton swab had a success rate of 84%, the balloon shape cotton swab 72%, and the cyto-brush 68%.
- Patient intolerance led to discontinuation in 32% of cases with cyto-brush, 28% with balloon shape swab, 16% with classical swab, and only 4% with urethral swab.
Conclusions:
- All tested instruments can obtain vaginal secretions in young children.
- The urethral cotton swab is the most effective and well-tolerated instrument for vaginal secretion sampling in pediatric vulvovaginitis cases.
- Optimizing sampling techniques is crucial for accurate diagnosis and appropriate management in pediatric gynecology.
