Purification of monoclonal antibodies by complex-displacement chromatography on CM-cellulose

A single high-capacity chromatographic procedure was used to purify a monoclonal antibody from ascites fluid. A new displacement procedure involving a displacer protein complex is described. The scale-up from 1 ml to 450 ml of ascites sample was easily accomplished with this complex-displacement system. A monoclonal antibody recovery of 79% was measured for the largest-scale purification.