Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid and simple method for purification of nucleic acids.

R Boom1, C J Sol, M M Salimans

  • 1Department of Virology, Academic Medical Center, Amsterdam, The Netherlands.

Journal of Clinical Microbiology
|March 1, 1990
PubMed
Summary

This study presents a fast and effective method for purifying DNA and RNA from biological samples like serum and urine using silica particles or diatoms. The protocol yields high amounts of nucleic acids suitable for downstream applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamics of colistin and tobramycin resistance among <i>Enterobacter cloacae</i> during prolonged use of selective decontamination of the digestive tract.

Antimicrobial resistance and infection control·2018
Same author

A misleading urethral smear with polymorphonuclear leucocytes and intracellular diplococci; case report of urethritis caused by Neisseria meningitidis.

Journal of medical microbiology·2013
Same author

Serogroup distribution of urogenital Chlamydia trachomatis in urban ethnic groups in The Netherlands.

Epidemiology and infection·2013
Same author

An abdominal mass: not a 'clear cut' case! Actinomycosis.

The Netherlands journal of medicine·2010
Same author

[Grounds for the inclusion of vaccination against cervical cancer within the National Immunisation Programme].

Nederlands tijdschrift voor geneeskunde·2008
Same author

[Vaccines against human papillomavirus (HPV); between registration and implementation].

Nederlands tijdschrift voor geneeskunde·2008

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Clinical Microbiology

Background:

  • Accurate nucleic acid purification is crucial for molecular diagnostics and research.
  • Existing methods can be time-consuming or require specialized equipment.
  • The need for rapid, small-scale purification from various biological fluids persists.

Purpose of the Study:

  • To develop a simple, rapid, and reliable protocol for small-scale DNA and RNA purification.
  • To utilize guanidinium thiocyanate and silica/diatoms for nucleic acid isolation.
  • To demonstrate the method's applicability across diverse sample types and sources.

Main Methods:

  • Utilized guanidinium thiocyanate for lysing and nuclease inactivation.
  • Employed size-fractionated silica particles or diatoms for nucleic acid binding.

Related Experiment Videos

  • Performed purification from human serum, urine, and bacterial cells.

    • Main Results:

    • Achieved purification of various DNA and rRNA types from 12 specimens in under 1 hour.
    • Obtained high yields (>50%) of purified DNA (picogram to microgram levels), suitable for enzymatic reactions.
    • Isolated microgram quantities of genomic DNA, plasmid DNA, and rRNA from cell-rich sources, including pathogenic bacteria.

    Conclusions:

    • The developed protocol offers a simple, rapid, and efficient method for DNA and RNA purification.
    • The technique is versatile, applicable to various nucleic acid types and biological specimens.
    • This method holds potential for widespread application in clinical microbiology and molecular diagnostics.