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Antifungal susceptibility testing method for resource constrained laboratories.

S Khan1, S Singhal, T Mathur

  • 1Department of Infectious Diseases, Ranbaxy Research Laboratories, Gurgaon - 122 001, Haryana, India. seema.khan@ranbaxy.com

Indian Journal of Medical Microbiology
|August 17, 2006
PubMed
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A new semisolid antifungal agar susceptibility (SAAS) method offers a simple and comparable alternative for antifungal susceptibility testing. This method is suitable for resource-constrained laboratories, providing reliable results for various fungal pathogens.

Area of Science:

  • Mycology
  • Clinical Microbiology
  • Antimicrobial Resistance

Background:

  • Antifungal susceptibility testing (AFST) is crucial for guiding treatment decisions.
  • Standard AFST methods like NCCLS/CLSI are often not feasible in resource-limited settings.
  • There is a need for simple, cost-effective AFST methods.

Purpose of the Study:

  • To describe and evaluate a simple semisolid antifungal agar susceptibility (SAAS) method.
  • To compare the SAAS method with standard NCCLS/CLSI methods for AFST.
  • To assess the feasibility of SAAS in resource-constrained laboratories.

Main Methods:

  • Determined reference Minimum Inhibitory Concentrations (MICs) using NCCLS/CLSI methods for 72 fungal isolates and two quality control strains against fluconazole, itraconazole, voriconazole, amphotericin B, and caspofungin.

Related Experiment Videos

  • Tested dermatophytes against terbinafine.
  • Optimized and performed AFST using the SAAS method in Brain Heart Infusion Agar (BHIA) without oil overlay, comparing results with NCCLS/CLSI methods.

    • Main Results:

    • The SAAS method yielded comparable results to NCCLS/CLSI methods for quality control strains.
    • MIC values obtained by SAAS for the 72 isolates did not vary by more than one dilution compared to reference methods for all tested antifungal drugs.
    • The SAAS method demonstrated reproducibility.

    Conclusions:

    • The SAAS method using BHIA without oil overlay is a simple and reproducible technique for AFST.
    • This method is suitable for determining MICs against yeasts, filamentous fungi, and dermatophytes.
    • SAAS is a viable option for antifungal susceptibility testing in resource-constrained laboratories.