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Related Experiment Videos

Surface plasmon resonance-enabled mass spectrometry arrays.

Dobrin Nedelkov1, Kemmons A Tubbs, Randall W Nelson

  • 1Intrinsic Bioprobes Inc., Tempe, AZ 85281, USA. dnedelkov@intrinsicbio.com

Electrophoresis
|August 18, 2006
PubMed
Summary

This study demonstrates a high-throughput biosensor approach combining surface plasmon resonance (SPR) and mass spectrometry (MS) for protein analysis. The developed SPR-MS arrays enable efficient capture and detection of specific proteins from complex samples like human plasma.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry

Background:

  • Surface plasmon resonance (SPR) biosensors offer sensitive detection of biomolecular interactions.
  • Mass spectrometry (MS) provides powerful qualitative and quantitative protein analysis.
  • Integrating SPR with MS (SPR-MS) enhances protein separation and analysis capabilities.

Purpose of the Study:

  • To develop a high-throughput SPR-MS approach using arrayed binding ligands for protein affinity capture.
  • To explore the feasibility of on-chip MS analysis of proteins captured by antibody arrays on SPR chips.

Main Methods:

  • Antibodies against specific proteins (beta-2-microglobulin, cystatin C, transferrin, IGFs) were arrayed on SPR chips.
  • Human plasma samples were analyzed using the SPR biosensor with antibody arrays.

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  • On-chip MS analysis was performed to detect proteins captured by the antibody arrays.

    • Main Results:

    • Successful antibody immobilization and specific protein capture were confirmed by SPR signals.
    • Targeted proteins were detected on each antibody-derivatized chip.
    • The SPR-MS array system demonstrated robustness, high reproducibility, and high-throughput capability.

    Conclusions:

    • The developed SPR-MS array technology enables efficient, multiplexed, and quantitative analysis of proteins.
    • This approach facilitates selective protein separation and subsequent MS identification.
    • The robust and reproducible SPR-MS arrays are suitable for high-throughput proteomic studies.