Quantitative analysis of phenobarbital in dosage form by thin-layer chromatography combined with densitometry
Related Experiment Video
Updated: Aug 6, 2026

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
Published on: October 6, 2022
Magdalena Wójciak-Kosior1, Agnieszka Skalska, Grazyna Matysik
1Medical Academy, Laboratory of Planar Chromatography, Department of Chemistry, Staszica 6, 20-081, Lublin, Poland. kosiorma@wp.pl
Abstract:
In this paper, a high-performance thin-layer chromatography (HPTLC) method combined with densitometry has been described. Chromatography was performed on silica gel Si 60F254 plates using dichloromethane-ethyl acetate-formic acid (9.5 +/- 0.5 +/- 0.1, v/v) mobile phase. This method has been successfully applied for the determination of phenobarbital in pharmaceuticals. Obtained results were comparable with traditionally used column high-performance liquid chromatography (HPLC) methods. For the proposed procedure, linearity (r > 0.999), sensitivity (limit of detection 0.4 microg/spot), recovery (97.8-102.1%), and repeatability were found to be satisfactory. The HPTLC-densitometry method has many advantages, such as simplicity, reasonable sensitivity, rapidity, and low cost, and it can be successfully used in routine quality control of multidrug preparations containing barbiturates.
More Related Videos
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
10:30A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Related Concept Videos
Therapeutic Drug Monitoring: Drug Analysis Methods
Protein-Drug Binding: Determination Methods
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
Dosage Regimens: Partial Pharmacokinetic Parameters