Microbiological assay for lomefloxacin in coated tablets

Area of Science:

• Pharmaceutical Sciences
• Microbiology
• Analytical Chemistry

Background:

• Lomefloxacin is an antibiotic used to treat bacterial infections.
• Accurate quantification of lomefloxacin in pharmaceutical formulations is essential for quality control.

Purpose of the Study:

• To validate a microbiological assay for determining lomefloxacin activity in coated tablets.
• To establish the reliability and accuracy of the cylinder plate method for lomefloxacin quantification.

Main Methods:

• The cylinder plate method was employed using Bacillus subtilis ATCC 9372 as the test organism.
• Lomefloxacin concentrations ranging from 2.0 to 8.0 microg/mL were analyzed.
• Method validation included assessments of linearity, precision, and accuracy.

Main Results:

• The microbiological assay demonstrated excellent linearity (r = 0.9999).
• The method exhibited high precision with a relative standard deviation of 1.15%.
• Accuracy was confirmed, as the assay correctly measured added quantities of lomefloxacin, and excipients did not interfere.

Conclusions:

• The validated microbiological assay is suitable for the accurate quantification of lomefloxacin in coated tablets.
• This method provides a reliable tool for pharmaceutical quality control of lomefloxacin formulations.
• The cylinder plate method offers a robust approach for antibiotic activity determination.