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Nucleic acid association to human prostasomes.

I Olsson1, G Ronquist

  • 1Pharmacia LKB AB, Uppsala, Sweden.

Archives of Andrology
|January 1, 1990
PubMed
Summary

Human prostasomes contain enriched nucleic acids, primarily double-stranded DNA, associated with their membranes. This association is genuine and not due to contamination, suggesting a functional role for nucleic acids in prostasomes.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Reproductive Biology

Background:

  • Prostasomes are vesicles secreted by prostate epithelial cells into seminal plasma.
  • Their composition and function remain incompletely understood, particularly regarding nucleic acid content.

Purpose of the Study:

  • To investigate the presence and nature of nucleic acids within human prostasomes.
  • To determine if nucleic acids are genuinely associated with prostasomes or represent contaminants.

Main Methods:

  • Phenol extraction and absorbance measurements (A260/A280) to quantify nucleic acids and proteins.
  • Sodium dodecyl sulfate (SDS) treatment and electrophoresis to assess nucleic acid dissociation.
  • Enzymatic degradation assays using DNases and RNases to identify nucleic acid types.

Main Results:

  • Prostasomes exhibit a higher nucleic acid to protein ratio (1:100) compared to seminal plasma (1:1,000), indicating nucleic acid enrichment.
  • Electrophoresis confirmed the dissociation of nucleic acids from prostasomes upon SDS treatment.
  • Enzymatic assays suggest the presence of double-stranded DNA, but not RNA, on the prostasome membrane surface.
  • Nucleic acids of varying sizes (200-20,000 bp) were associated with prostasomes, ruling out common contaminants.

Conclusions:

  • Human prostasomes contain significantly enriched nucleic acids, predominantly double-stranded DNA.
  • The association of DNA with prostasome membranes is genuine and likely functional, not a result of contamination.
  • Further research is warranted to elucidate the specific role of prostasomal DNA.

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