Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hypermethylation of RASSF1A in human and rhesus placentas.

The American journal of pathology·2007
Same author

Ubiquitous aberrant RASSF1A promoter methylation in childhood neoplasia.

Clinical cancer research : an official journal of the American Association for Cancer Research·2004
Same author

Tumor-derived aberrant methylation in plasma of invasive ductal breast cancer patients: clinical implications.

Oncology reports·2003
Same author

The sporadic nature of shedding cells in blood: Multiple RNA diagnostic testing and prognostication of cancer progression.

Clinical chemistry·2003
Same author

Quantitative analysis of tumor-derived methylated p16INK4a sequences in plasma, serum, and blood cells of hepatocellular carcinoma patients.

Clinical cancer research : an official journal of the American Association for Cancer Research·2003
Same author

Transcriptional profiling of circulating tumor cells: quantification and cancer progression (Review).

Oncology reports·2002

Related Experiment Video

Updated: Jul 15, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Qualitative and quantitative polymerase chain reaction-based methods for DNA methylation analyses.

Ivy H N Wong1

  • 1Department of Obstetrics and Gynaecology, Prince of Wales Hospital, The Chinese University of Hong Kong, SAR.

Methods in Molecular Biology (Clifton, N.J.)
|August 19, 2006
PubMed
Summary

DNA methylation analysis is efficiently performed using various qualitative and quantitative polymerase chain reaction (PCR)-based methods. Methylation-specific PCR (MSP) offers a fast, sensitive, and specific approach for these crucial DNA methylation analyses.

More Related Videos

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Related Experiment Videos

Last Updated: Jul 15, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain
13:11

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • DNA methylation is a key epigenetic modification regulating gene expression.
  • Accurate analysis of DNA methylation patterns is essential for understanding various biological processes and diseases.

Purpose of the Study:

  • To review and summarize various polymerase chain reaction (PCR)-based methods for analyzing DNA methylation.
  • To highlight the advantages and applications of different techniques.

Main Methods:

  • Methylation-specific PCR (MSP)
  • Bisulfite sequencing
  • Methylation-sensitive restriction enzyme PCR
  • Combined bisulfite restriction analysis (COBRA)
  • Methylation-sensitive single nucleotide primer extension (Ms-SNuPE)
  • Quantitative real-time MSP

Main Results:

  • PCR-based methods provide easy qualitative and quantitative analysis of DNA methylation.
  • MSP is fast, sensitive, and specific, coupling bisulfite modification with PCR.
  • Quantitative methods like COBRA, Ms-SNuPE, and real-time MSP enable precise methylation level determination.

Conclusions:

  • A range of PCR-based techniques are available for effective DNA methylation analysis.
  • The choice of method depends on the specific requirements for qualitative or quantitative assessment.
  • These methods are crucial tools in epigenetic research and diagnostics.