Related Experiment Video

Updated: Aug 6, 2026

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
09:57

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

The potential of the tumor microenvironment to influence Apo2L/TRAIL induced apoptosis

Thomas A Mace1, Nariyuke Yamane, Jinrong Cheng

  • 1Department of Immunology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.

Immunological Investigations
|August 19, 2006
PubMed

Insights

Apo2L/TRAIL induces apoptosis in many cancer cells, inhibiting tumor growth in patient xenografts. Understanding resistance factors in the tumor microenvironment is key for effective cancer therapy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Immunology

Background:

  • Apo2L/TRAIL induces apoptosis in malignant cells via DR4/DR5 receptors, sparing normal cells.
  • Previous studies demonstrated anti-tumor effects in vitro and in vivo xenografts.
  • Tumor microenvironment factors can influence Apo2L/TRAIL resistance.

Purpose of the Study:

  • To review factors influencing patient tumor response to Apo2L/TRAIL.
  • To explore the efficacy of Apo2L/TRAIL against patient-derived xenografts.
  • To highlight the clinical potential of targeting DR4 and DR5 receptors.

Main Methods:

  • Investigated Apo2L/TRAIL efficacy in SCID mouse models with patient tumor xenografts.
  • Reviewed literature on tumor microenvironment factors affecting Apo2L/TRAIL response.
  • Examined resistance mechanisms to Apo2L/TRAIL-induced apoptosis.

Main Results:

  • Apo2L/TRAIL inhibited the growth of primary and metastatic patient tumors in xenografts.
  • Identified hypoxia, MMPs, and cytokines as potential modulators of Apo2L/TRAIL efficacy.
  • Confirmed Apo2L/TRAIL's selective toxicity towards malignant cells.

Conclusions:

  • Apo2L/TRAIL shows promise as an anti-cancer therapeutic, particularly against patient tumors.
  • Further research into tumor microenvironment interactions is crucial for optimizing Apo2L/TRAIL therapy.
  • Targeting DR4 and DR5 receptors holds significant clinical potential for cancer treatment.

More Related Videos

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments07:46

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments

Published on: April 30, 2021

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis09:42

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

Published on: June 26, 2019

Related Concept Videos

The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...