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Updated: Aug 6, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
Genetic variability of bovine viral diarrhoea virus subtypes at 3'-nontranslated region
S Vilcek1, Mária Kolesárová, Anna Jacková
1Department of Infectious Diseases and Parasitology, University of Veterinary Medicine, Komenskeho 73, 041 81, Kosice, Slovakia. vilcek@uvm.sk
Abstract:
Ten isolates belonging to different BVDV-1 subtypes and three BVDV-2 isolates were studied in 3'-nontranslated region (3'-NTR) by sequencing of PCR products and comparative computer-assisted nucleotide sequence analysis. The alignment of nucleotide sequences revealed that all BVDV-1 isolates except BVDV-1a isolates represented by NADL strain were characterised with a deletion of 38 nucleotides in the variable region located after the stop codon. For all BVDV-1 subtypes, a constant region at the end of viral genome was highly conserved. The variable and constant regions with no significant insertions or deletions were also identified in BVDV-2 isolates. The poly AT reach region was situated at different locations in both pestiviruses.
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