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RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
Published on: September 28, 2011
Bacterial RNA isolation with one hour centrifugation in a table-top ultracentrifuge
K J Reddy1, R Webb, L A Sherman
1Dept. of Biological Sciences, Purdue University, West Lafayette, IN 47907.
Biotechniques
|March 1, 1990
Abstract:
A procedure for the rapid preparation of cesium-chloride purified RNA from E. coli and the cyanobacterium Synechococcus sp. PCC7942 is described. Cells are lysed in modified sucrose, Triton X-100, EDTA, Tris buffer with phenol/chloroform. The cleared lysate is extracted further with phenol/chloroform and RNA is peleted by centrifugation through a 5.7 M CsCl cushion. High quality RNA can be prepared in three hours using this procedure.
