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Protamine and mast-cell-mediated angiogenesis in the rat
A Jakobsson1, J Sörbo, K Norrby
1Department of Pathology, Gothenburg University, Sahlgren's Hospital, Sweden.
Journal of Experimental Pathology (Oxford, England)
|April 1, 1990
Summary
Protamine sulfate (PS) was tested for toxicity and its effect on blood vessel formation in rats. PS demonstrated angiostatic effects at a specific dose, but not a general anti-angiogenesis impact.
Area of Science:
- Pharmacology
- Angiogenesis Research
- Toxicology
Background:
- Protamine sulfate (PS) is used clinically, but its non-specific toxic effects require investigation.
- Mast cells play a role in inflammatory processes and angiogenesis.
Purpose of the Study:
- To evaluate the non-specific toxicity of different doses of protamine sulfate (PS).
- To assess the effect of PS on mast cell-mediated angiogenesis in rats.
Main Methods:
- Rats received subcutaneous injections of varying PS doses.
- Mast cell secretion and angiogenesis were induced using Compound 48/80.
- Angiogenesis was quantified by counting blood vessels in mesenteric window sections.
Main Results:
- No clear dose-effect relationship was observed for PS on angiogenesis.
- A statistically significant suppression of angiogenesis occurred only at 40 mg PS/kg, not at 20 or 60 mg/kg.
- PS did not exhibit general toxicity concerning body weight or gut proliferation.
Conclusions:
- Protamine sulfate can exhibit angiostatic properties.
- PS does not exert a broad angiostatic effect in the studied rat model.