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Related Experiment Videos

Mapping pharmaceuticals in tissues using MALDI imaging mass spectrometry.

Yunsheng Hsieh1, Jiwen Chen, Walter A Korfmacher

  • 1Drug Metabolism and Pharmacokinetics Department, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA. yunsheng.hsieh@spcorp.com

Journal of Pharmacological and Toxicological Methods
|August 22, 2006
PubMed
Summary

Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) offers a new way to visualize drug distribution in tissues. This method overcomes limitations of radioautography and HPLC-MS/MS for drug discovery and development.

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Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Biotechnology

Background:

  • Assessing drug distribution to the site of action is crucial in drug discovery.
  • High-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) is widely used but has limitations.
  • Whole body radioautography (WBA) visualizes total drug-related materials but not specific drug or metabolite distribution.

Purpose of the Study:

  • To introduce matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) as a novel method for drug distribution analysis.
  • To overcome the limitations of existing techniques like WBA and HPLC-MS/MS in drug development.
  • To enable direct visualization of drug and metabolite distribution in tissue sections.

Main Methods:

  • Development and application of matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS).

Related Experiment Videos

  • Direct determination of pharmaceutical distribution in tissue sections.
  • Comparison with traditional methods like HPLC-MS/MS and WBA.

    • Main Results:

    • MALDI-MS allows direct visualization of pharmaceutical distribution in tissue sections.
    • This technique can potentially unravel drug disposition and biotransformation pathways.
    • Overcomes limitations of WBA regarding visualization of specific compounds and the need for radiolabeled drugs.

    Conclusions:

    • MALDI-MS is a powerful tool for determining drug distribution in tissues during drug discovery.
    • It provides insights into drug disposition and biotransformation, aiding pharmaceutical development.
    • MALDI-MS offers advantages over conventional methods for visualizing drug distribution in preclinical studies.