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[Comparison the screening methods of the acute toxicity in vitro]
Zheng Wang1, Tian-Bao Zhang, Yu-Ping Zhu
1Department of Toxicology, Second Military Medical University, Shanghai 200433, China.
Objective:
To provide the proof for establishing the high throughput screening method of the acute toxicity by comparing the 4 screening methods of the acute toxicity in vitro.
Methods:
HepG2 cells, plated in 96-well plates, were treated with 47 compounds and we detected the cytotoxicity using 4 methods and calculated the IC50, IC45, IC40, IC35, IC30, and these values were compared with the LD50.
Results:
The IC45 from the neutral red method has the best relativity with the LD50.
Conclusion:
Plating HepG2 cells in 96-well plates, the cytotoxicity be detected using the neutral red method and the IC45 was calculated. It is considered to provide the proof for establishing the high throughput screening method of the acute toxicity in vitro.
