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Updated: Aug 6, 2026
Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
[A study of the determination of H2O2 in solid food by enzyme-oxygen electrode]
Ying Xue1, Ping Liu, Tai-Ran Liu
1Center for Disease Control and Prevention of Beijing, Beijing 100013, China.
Objective:
A oxygen-electrode method is developed for the determination of H2O2 residues in solid food.
Methods:
The hydrogen peroxide can be decomposed specifically into water and oxygen by catalase. The oxygen-electrode has high selectivity with the dissolved oxygen (DO). After H2O2 in the samples is extracted by PBS and the system is deoxidized by nitrogen, the DO can be detected by oxygen meter and then the content of H2O2 in samples will be calculated according to the result.
Results:
The linear range is from 0.5 microg/ml to 7.5 microg/ml r > 0.999. The detection limit is 0.13 microg/ml. The recoveries are between 90.59%-93.88%. BSD is below 10%.
Conclusion:
The method has been used for the detection of H2O2 in health-preservation products of calcium, dried small shrimps, bean curd et al, it proved to be a simple rapid method.
