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Published on: September 5, 2017
[Studies on the mechanism of Cr-induced DNA damage in glouse]
Li-Wei Shi1, Hai-Ning Jing, Gai-Ying Ren
1Institute of Environmental Health and Related Product Safety, China CDC, Beijing 100021, China.
Objective:
To investigate the mechanism of Cr-induced DNA damage in glouse.
Methods:
The studies were conducted at three pH values(pH 6.5, 7.5, 8.5) with a range of sugars, including D-glucose, alpha-methyl-D-glucose, 2-deoxy-D-glucose, and D-glucose-C-d7. DNA damage was analyzed by gel electrophoresis, and the new species and their lifetime were detected by EPR.
Results:
The results of these experiments have reinforced the postulate that Cr (V) complexes are likely to be involved in the genotoxic damage that eventually results in Cr-induced cancers. With an increase in the pH values, the amount of Form II DNA decreased due to the increasing rate of Cr (V) disproportionation and H concentration. On the other hand, the presence of sugars in the reaction system generated Cr-mixed-ligand complexes, which could also cause DNA cleavage.
Conclusion:
Proposed mechanism of DNA cleavage are presented induced by Cr (V)-mixed-ligand.
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