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Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
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Detection and quantification of Renibacterium salmoninarum DNA in salmonid tissues by real-time quantitative

Dorothy M Chase1, Diane G Elliott, Ronald J Pascho

  • 1US Geological Survey, Biological Resources Discipline, Western Fisheries Research Center, 6505 N.E. 65th Street, Seattle, WA 98115, USA.

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
|August 23, 2006
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...