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A quantitative assay for tyrosine sulfation and tyrosine phosphorylation in peptides
H Blode1, T Heinrich, H Diringer
1Robert Koch-Institut des Bundesgesundheitsamtes, Berlin.
Biological Chemistry Hoppe-Seyler
|February 1, 1990
Abstract:
A method was developed to measure sulfation and phosphorylation of tyrosine in proteins after alkaline hydrolysis, ion-exchange chromatography, reaction with [3H]dinitrofluorobenzene and subsequent thin-layer chromatography. The method allows the detection of 10-20 pmol of modified tyrosine and was applied to determine the content of tyrosine-phosphate and -sulfate in fibrinogens, thyroglobulin, alpha-casein, cytochrome c and glyceraldehyde dehydrogenase.