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CD5 (Ly-1)-negative, conventional splenic B cells make a substantial contribution to the bromelain plaque-forming
1Division of Clinical Immunology, Kennedy Institute of Rheumatology, London, U.K.
Immunology
|April 1, 1990
Abstract:
CD5 (Ly-1) B cells are a minor subpopulation in mouse spleen and are thought to be responsible for the production of natural autoantibodies to bromelain-treated autologous erythrocytes (Br-RBC). Here it is shown that substantial numbers of conventional, CD5-negative, splenic B cells also secrete these antibodies in CBA and (NZB x NZW)F1 mice, whereas in NZB and BALB/c mice they are all produced by the CD5 B-cell population. However, stimulation with bacterial lipopolysaccharide in vivo preferentially activates the CD5 B-cell group to anti-Br-RBC antibody secretion.