Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human epithelial-specific response to pathogenic Campylobacter jejuni.

Erica S Rinella1, Chevonne D Eversley, Ian M Carroll

  • 1Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA.

FEMS Microbiology Letters
|August 23, 2006
PubMed
Summary

Human and mouse gut cells respond differently to bacteria. The human pathogen Campylobacter jejuni specifically alters human colonic cell gene expression, impacting growth and cell functions linked to gastrointestinal disease.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Almond Meal-Rich Diet Improves Red Blood Cell Count and Reduces Odds of Anemia in a Mouse Model of Aging.

Antioxidants (Basel, Switzerland)·2026
Same author

Non-Mendelian inheritance of DNA methylation patterns in mice.

Nature genetics·2026
Same author

The association between virus-induced spinal cord pathology and the genetic background of the host.

Journal of neuropathology and experimental neurology·2025
Same author

Strain and sex effects on blood accumulation of lead, chromium, and cadmium in strains from the Collaborative Cross mouse population.

bioRxiv : the preprint server for biology·2025
Same author

Exploring the Effects of Dietary Intake on Gut Microbiome Diversity and Composition of Older Adults with Type 1 Diabetes: A Pilot Study.

Current developments in nutrition·2025
Same author

Derivation of ultrasound and electrocardiogram cardiac reference ranges for Mus musculus using the collaborative cross and identification of new cardiac models.

BMC cardiovascular disorders·2025

Area of Science:

  • Gastroenterology
  • Microbiology
  • Immunology

Background:

  • Mammalian gastrointestinal epithelia tolerate commensal gut microbes but react strongly to pathogens.
  • Host-specific responses to gastrointestinal pathogens are not well understood.

Purpose of the Study:

  • To compare the transcriptional responses of human and murine colonic epithelial cells to commensal and pathogenic bacteria.
  • To identify host-specific gene expression changes induced by Campylobacter jejuni.

Main Methods:

  • Transcriptional profiling of cultured human (Caco-2) and murine (CT-26) colonic epithelial cells.
  • Exposure of cells to Lactobacillus reuteri (commensal) and Campylobacter jejuni (pathogen).
  • Differential gene expression analysis, accounting for bacterial broth effects.

Related Experiment Videos

Main Results:

  • Both bacterial species induced stronger gene expression changes in human cells compared to mouse cells in standard broth.
  • After correcting for broth effects, only human cells exposed to C. jejuni showed significant gene expression alterations.
  • Affected genes in human cells were related to growth, transcription, steroid biosynthesis, cell polarity, and water transport.

Conclusions:

  • Mouse and human colonic epithelia are largely unresponsive to commensal bacteria.
  • Campylobacter jejuni elicits a distinct, host-specific transcriptional response in human colonic epithelia.
  • C. jejuni-induced changes in human genes linked to cell polarity and water transport may connect infection to gastrointestinal disease.