Involvement of mesangial cells expressing alpha-smooth muscle actin during restorative glomerular remodeling in...

The function of actin cytoskeleton in mesangial cells (MCs) during the recovering process of injured glomeruli is not fully understood. MCs in injured glomeruli express alpha-smooth muscle actin (alpha-SMA), which is not detected in normal glomeruli. We focused on the localization of alpha-SMA in MCs of Thy-1.1 nephritic rat. Expression of alpha-SMA in the injured glomeruli peaked at day 5 after antibody injection and then declined gradually. At day 5, MCs, where alpha-SMA was localized at their cytoplasmic processes situated in various positions, occupied the expanded mesangium. MCs expressing alpha-SMA tended to be located at the peripheral region close to the glomerular basement membrane (GBM) or endothelial cells at day 8. Localization of alpha-SMA within the peripheral MCs was restricted to the cytoplasmic processes radiating toward the GBM and touching it with their tips at day 8. These alpha-SMA-containing processes are suitable to transmit the contractile force to GBM and may contribute to normalize the expanded glomerular volume. In addition, an actin-binding protein, drebrin, was localized in all MC processes extending toward various directions throughout the course of nephritis, suggesting that drebrin is involved in the formation of MC processes.