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[Effect of chronic transauricular kindled seizures on passive-avoidance test memory retention in rats]
Qing Li1, Lu-ying Liu, Yuan-yuan Zhu
1Department of Pharmacology, College of Medicine, Zhejiang University, Hangzhou 310031, China.
Objective:
To investigate effect of chronic transauricular kindled seizures on passive-avoidance test memory retention in rats.
Methods:
Chronic transauricular kindled seizures was induced by repeated application of initially subconvulsive electrical stimulation through ear-clip electrodes once every 24 h until the occurrence of 3 consecutive clonic-tonic seizures. A passive avoidance test was used to measure memory retention ability. Morphological changes in neurons of hippocampal CA1 region was examined after HE staining. Histamine, gamma-aminobutyric acid (GABA) and glutamate levels in the hippocampus were measured by high performance liquid chromatography (HPLC).
Result:
Chronic transauricular kindled seizures impaired passive-avoidance test memory retention in rats. The damaged CA1 neurons were observed and histamine content in the hippocampus markedly decreased 24 h after the end of kindling in the chronic transauricular kindled rats.
Conclusion:
Chronic transauricular kindled seizure impaired passive-avoidance test memory retention, and it might be due to the damaged CA1 neurons and a decrease of histamine in the hippocampus induced by epilepsy.
