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Interstitial cells and phasic activity in the isolated mouse bladder.

Magdalini Lagou1, Marcus J Drake, Marjanne Markerink-VAN Ittersum

  • 1The Urophysiology Research Group, School of Surgical and Reproductive Sciences, The Medical School, The University, Newcastle upon Tyne, UK.

BJU International
|August 24, 2006
PubMed
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Interstitial cells (ICs) in the mouse bladder respond to nitric oxide (NO) by increasing cyclic guanosine monophosphate (cGMP). Activating these NO/cGMP-sensitive ICs modulates bladder contractile activity, suggesting a functional role in generating and controlling phasic contractions.

Area of Science:

  • Urology
  • Neuroscience
  • Cell Biology

Background:

  • Interstitial cells (ICs) are crucial for smooth muscle function.
  • Nitric oxide (NO) plays a significant role in regulating bladder activity.
  • The specific distribution and function of NO-sensitive ICs in the bladder remain incompletely understood.

Purpose of the Study:

  • To map the distribution of NO-sensitive interstitial cells (ICs) in the mouse bladder.
  • To investigate the effect of NO-donor exposure on bladder contractile activity.

Main Methods:

  • Immunohistochemistry was used to visualize cGMP in bladder tissue after NO-donor incubation.
  • Intravesical pressure was recorded in isolated mouse bladders.
  • Diethylamine NONOate (NO donor) and muscarinic agonists were applied to assess effects on bladder pressure.

Related Experiment Videos

Main Results:

  • NO-sensitive ICs were identified in the outer muscle layers of the mouse bladder, categorized as surface muscle ICs and intramuscular ICs.
  • Cholinergic nerve fibers co-localized with neuronal NO synthase (nNOS) and showed increased cGMP in response to NO.
  • Exposure to a NO donor abolished muscarinic-induced phasic bladder activity, suggesting modulation of contractile patterns.

Conclusions:

  • The study confirms the presence of NO/cGMP-sensitive ICs in the outer muscle layers of the mouse bladder.
  • Activation of these ICs influences muscarinic-induced phasic bladder contractions.
  • These findings suggest a novel functional role for ICs in generating and modulating bladder phasic activity.