Related Experiment Video
Updated: Jul 20, 2026

Investigations on Alterations of Hippocampal Circuit Function Following Mild Traumatic Brain Injury
Published on: November 19, 2012
Characterization of rebound depolarization in hippocampal neurons
Rainer Surges1, Monika Sarvari, Marc Steffens
1Department of Neurology and Clinical Neurophysiology, University Clinics of Freiburg, Germany. rainer.surges@uniklinik-freiburg.de
Abstract:
Rebound depolarization (RD) following hyperpolarizing pulses is found in several neuronal cell types where it takes part in the regulation of neuronal firing behavior. During whole-cell current and voltage clamp recordings in slice preparations, we investigated the modulation of RD by different stimulation patterns and its underlying ionic currents in rat CA1 pyramidal cells. RD was mainly carried by the hyperpolarization-activated cation current I(h) (about two-third) and T-type calcium currents (about one-third), respectively. RD increased with increasing hyperpolarizing amplitude and stimulation frequency, whereas RD substantially decreased with longer pulse duration and, less pronounced, with increasing pulse number. The pulse duration-related decrease of RD was due to a decrease of the driving force of I(h). In conclusion, we showed that RD is differentially modulated by precedent hyperpolarization. Since RD amplitude was high enough to generate action potentials, RD may serve, even under physiologic conditions, as an inhibition-excitation converter.
More Related Videos
09:51Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
Published on: January 1, 2018
16:38Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
Published on: November 26, 2012
Related Concept Videos
Action Potential: Phases of Stimulation
Resting Phase:
In this phase, the cell's membrane is at its resting potential, typically around -70 millivolts (mV) for neurons. Inside the cell, there is a higher concentration of potassium ions (K+) and a lower concentration of sodium ions (Na+). Voltage-gated sodium channels are closed, and...
Resting Potential Decay
At rest, the K+ is the main ion that moves across the membrane through...
The Role of Ion Channels in Neuronal Computation
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential.
Action Potentials
Action Potential
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...
Action Potential
Membrane potential in neurons
Neurons typically have a resting membrane potential of about -70 millivolts (mV). When they receive...