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Updated: Jul 20, 2026
08:31
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
Published on: June 8, 2018
The cellular control of DNA double-strand breaks
1Department of Radiation Oncology, Washington University School of Medicine, 4511 Forest Park, St. Louis, MO 63108, USA.
Journal of Cellular Biochemistry
|August 24, 2006
Summary
DNA double-strand breaks (DSBs) are dangerous genome lesions. Eukaryotic cells possess repair mechanisms to maintain genomic integrity and prevent cancer, involving signaling pathways and posttranslational modifications.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- DNA double-strand breaks (DSBs) are highly toxic genomic lesions.
- DSBs occur naturally during vital cellular processes like replication and meiosis.
- Efficient DSB repair is critical for genomic integrity, cell survival, and cancer prevention.
Purpose of the Study:
- To review the mechanisms controlling DNA double-strand breaks in eukaryotic cells.
- To discuss physiological processes that necessitate DSB formation.
- To explore how DSBs are managed in both damaged and undamaged cells.
Main Methods:
- Review of existing literature on DNA double-strand break response.
- Analysis of signaling pathways involved in DSB sensing and repair.
- Examination of posttranslational modifications in DSB effector functions.
Main Results:
- Eukaryotic cells have evolved sophisticated systems to detect and respond to DSBs.
- A hierarchical signaling cascade transmits the damage signal to downstream effectors.
- Posttranslational modifications, including phosphorylation and acetylation, are key to effector function.
Conclusions:
- The cellular response to DSBs is rapid and involves complex signaling networks.
- Understanding DSB control is essential for comprehending genome maintenance and disease prevention.
- This review consolidates knowledge on DSB management in various cellular contexts.
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