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Deoxyribonuclease zymography adapted to the precast PhastGel electrophoresis media
1Department of Biochemistry, University of Bergen, Norway.
Electrophoresis
|April 1, 1990
Abstract:
A set-up for casting fluorescent indicator agarose gels on ultrathin polyacrylamide microelectrophoresis gel media (Pharmacia PhastGel media) is described. The zymogram system allows a rapid and sensitive detection of deoxyribonuclease in various gel media, following isoelectric focusing, native and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.