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Updated: Jul 20, 2026
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Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
[Aggregation after rehydration of lyophilized platelets]
Jing-Han Liu1, Fa-Qiang Lu, Yuan Zhuang
1Department of Blood Transfusion, Center for Clinical Transfusion Medicine, The General Hospital of Chinese PLA, Beijing, 100853, China. jinghan-liu@301hospital.com.cn
Zhongguo Shi Yan Xue Ye Xue Za Zhi
|August 25, 2006
Summary
Lyophilized platelets show reduced aggregation compared to fresh platelets. However, trehalose, both intracellular and extracellular, significantly enhances the aggregation of rehydrated lyophilized platelets.
Area of Science:
- Biotechnology
- Hematology
- Biochemistry
Context:
- Platelet preservation is crucial for transfusion medicine.
- Lyophilization offers a long-term storage solution but can impair platelet function.
- Rehydrated lyophilized platelets exhibit altered aggregation properties.
Purpose:
- To investigate the aggregation capacity of rehydrated lyophilized platelets.
- To determine the optimal concentrations for platelet aggregation inducers.
- To evaluate the effect of trehalose on rehydrated lyophilized platelet aggregation.
Summary:
- Fresh platelets aggregated at approximately 100%, while rehydrated lyophilized platelets showed lower rates with thrombin, ristocetin, ADP, and collagen.
- Optimal inducer concentrations were identified as thrombin (1 U/ml), ristocetin (1.6 mg/ml), ADP (20 µmol/L), and collagen (2 µg/ml).
- Both intracellular and extracellular trehalose significantly improved the maximum aggregation rate of rehydrated lyophilized platelets compared to controls.
Impact:
- Establishes optimal conditions for assessing rehydrated lyophilized platelet aggregation.
- Demonstrates trehalose's potential as a cryoprotectant to improve platelet function post-lyophilization.
- Suggests strategies for enhancing the viability and efficacy of stored platelets.
