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A radioreceptor assay for follicle-stimulating hormone
The Journal of Clinical Endocrinology and Metabolism
|September 1, 1975
Summary
A new radioreceptor assay for follicle-stimulating hormone (FSH) offers high specificity and sensitivity for measuring FSH in serum and pituitary samples. This assay provides precise quantification and distinguishes FSH from other hormones.
Area of Science:
- Endocrinology
- Biochemistry
- Assay Development
Background:
- Follicle-stimulating hormone (FSH) is crucial for reproductive function.
- Accurate measurement of FSH is essential for diagnosing endocrine disorders.
- Existing assays may lack the required specificity or sensitivity.
Purpose of the Study:
- To develop a highly specific and sensitive radioreceptor assay for FSH.
- To validate the assay's performance for measuring FSH in biological samples.
- To compare the assay's performance with existing methods.
Main Methods:
- Development of a radioreceptor assay using bovine testicular membranes.
- Radioiodination of human FSH (hFSH) using the lactoperoxidase method.
- Validation of assay specificity, sensitivity, and precision using purified hormones and biological samples.
Main Results:
- The assay demonstrated a detection limit of 1 ng/ml for purified hFSH and 2.5 ng/ml for serum.
- High specificity was observed, with minimal cross-reactivity from luteinizing hormone (LH) and thyroid-stimulating hormone (TSH).
- Potency estimations for purified FSH from various species were determined, and the index of discrimination between radioimmunoassay and radioreceptor assay was calculated for pituitary and serum FSH.
Conclusions:
- A novel, highly specific, and sensitive radioreceptor assay for FSH has been successfully developed.
- The assay is suitable for quantifying FSH in both pituitary tissue and serum.
- The assay provides valuable insights into FSH quantification and its discrimination from other hormones.