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Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Cleavage and Blastulation01:33

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After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
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Zygotic Development And Stem Cell Formation01:10

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The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
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Source And Potency Of Stem Cells01:27

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Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
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Embryonic Stem Cells00:57

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Derivation of Human Embryonic Stem Cells by Immunosurgery
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Human embryonic stem cell lines derived from single blastomeres.

Irina Klimanskaya1, Young Chung, Sandy Becker

  • 1Advanced Cell Technology, 381 Plantation Street, Worcester, Massachusetts 01605, USA.

Nature
|August 25, 2006
PubMed
Summary
This summary is machine-generated.

Human embryonic stem (hES) cells can now be derived from single blastomeres, a method compatible with preimplantation genetic diagnosis (PGD). This breakthrough offers a way to generate stem cell lines without embryo destruction.

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Area of Science:

  • Reproductive biology
  • Stem cell research
  • Genetics

Background:

  • Current human embryonic stem (hES) cell derivation necessitates embryo destruction.
  • Preimplantation genetic diagnosis (PGD) involves single-cell embryo biopsy, potentially sparing embryos.

Purpose of the Study:

  • To demonstrate the feasibility of deriving hES cells from single blastomeres.
  • To establish a method for stem cell generation that avoids embryo destruction.

Main Methods:

  • Micromanipulation techniques for single blastomere biopsy from human embryos.
  • Culturing biopsied blastomeres to derive stem cell lines.
  • Characterization of derived stem cell lines for pluripotency and stability.

Main Results:

  • Successful derivation of two stable hES cell lines from single blastomeres.
  • Derived hES cells maintained undifferentiated proliferation for over eight months.
  • Confirmed pluripotency and normal karyotype of the derived hES cells, with potential for differentiation into all three germ layers.

Conclusions:

  • hES cells can be derived from single blastomeres, offering an alternative to embryo destruction.
  • This method supports ethical stem cell generation and potential for patient-matched therapies.
  • The technique is compatible with PGD, allowing for simultaneous genetic testing and stem cell derivation.