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Updated: Jul 20, 2026

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Virus discovery by sequence-independent genome amplification
Helen E Ambrose1, Jonathan P Clewley
1Virus Reference Department, Centre for Infections, Health Protection Agency, London NW9 5EQ, UK.
Abstract:
Genome sequences from several blood borne and respiratory viruses have recently been recovered directly from clinical specimens by variants of a technique known as sequence-independent single primer amplification. This and related methods are increasingly being used to search for the causes of diseases of presumed infectious aetiology, but for which no agent has yet been found. Other methods that do not require prior knowledge of the genome sequence of any virus that may be present in the patient specimen include whole genome amplification, random PCR and subtractive hybridisation and differential display. This review considers the development and application of these techniques.
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