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Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy
Published on: October 6, 2014
Intestinal microbiota: a potential diet-responsive prevention target in ApcMin mice
Volker Mai1, Lisa H Colbert, Susan N Perkins
1Department of Epidemiology and Preventive Medicine, The University of Maryland School of Medicine, Baltimore, Maryland 21201-1192, USA.
Molecular Carcinogenesis
|August 25, 2006
Summary
Dietary interventions, including calorie restriction (CR) and a fruit/vegetable extract diet (OFV), reduced intestinal tumors in mice. Specific gut bacteria, Lachnospiraceae, were associated with this protective effect, indicating their potential as biomarkers.
Area of Science:
- Gastroenterology
- Microbiology
- Oncology
Background:
- Dietary interventions like calorie restriction (CR) and specific diets (OFV) previously showed reduced intestinal adenomas in Apc(Min) mice.
- The OFV diet's high fiber content influences gut microbiota composition, suggesting a role in diet-mediated effects on intestinal carcinogenesis.
Purpose of the Study:
- To investigate how CR and OFV diets alter intestinal microbiota composition.
- To identify specific bacterial changes associated with reduced intestinal carcinogenesis in Apc(Min) mice.
Main Methods:
- Fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) were used to analyze intestinal microbiota composition.
- 16S rDNA sequencing was employed to identify bacterial species associated with reduced polyp development.
Main Results:
- The OFV diet significantly altered intestinal microbiota composition, while CR induced smaller changes.
- A 16S rDNA fragment from previously unidentified Lachnospiraceae bacteria was detected in mice with reduced intestinal tumors.
Conclusions:
- Dietary interventions modulate the intestinal microbiota in Apc(Min) mice.
- Lachnospiraceae bacteria may serve as an indicator of intestinal conditions associated with reduced carcinogenesis.
