Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Early Multimodal Motor Training After Stroke Promotes Motor Recovery and Whole-Brain Structural Remodeling.

Journal of the American Heart Association·2026

Same author

Impact of an online-guided physical activity intervention on cognition, resting-state brain connectivity, and the gut microbiome in healthy older adults-a randomized controlled trial.

GeroScience·2026

Same author

Physical Activity and BrainAGE: Exploring the Impact on Brain Health and Plasticity in Older Adults.

Human brain mapping·2025

Same author

Metabolic modelling reveals the aging-associated decline of host-microbiome metabolic interactions in mice.

Nature microbiology·2025

Same author

Heterogeneity of ventricular action potentials in neonatal rat cardiomyocytes and methodological aspects of patch clamp measurements.

Frontiers in physiology·2025

Same author

Targeted rescue of synaptic plasticity improves cognitive decline in sepsis-associated encephalopathy.

Molecular therapy : the journal of the American Society of Gene Therapy·2024

Search research articles

Related Experiment Video

Updated: Jul 20, 2026

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

Microarray-based long-term detection of genes differentially expressed after cortical spreading depression.

Anja Urbach¹, Claus Bruehl, Otto W Witte

¹Department of Neurology, Friedrich-Schiller-University, Erlanger Allee 101, 07747 Jena, Germany. anja.urbach@med.uni-jena.de

The European Journal of Neuroscience

|August 26, 2006

Summary

Repetitive spreading depression (SD) in rats alters gene expression for up to 30 days, potentially promoting cortical plasticity and tolerance to ischemia. This study reveals a dynamic temporal pattern of genetic changes following SD events.

More Related Videos

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Published on: June 13, 2011

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Published on: April 10, 2013

Related Experiment Videos

Last Updated: Jul 20, 2026

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Mining Spatial Transcriptomics Datasets using DeepSpaceDB

Published on: September 5, 2025

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Modeling Neural Immune Signaling of Episodic and Chronic Migraine Using Spreading Depression In Vitro

Published on: June 13, 2011

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Laser Capture Microdissection of Enriched Populations of Neurons or Single Neurons for Gene Expression Analysis After Traumatic Brain Injury

Published on: April 10, 2013

Area of Science:

Neuroscience
Molecular Biology
Genetics

Background:

Spreading depression (SD) is a wave of neuronal depolarization implicated in neurological conditions like migraine and stroke.
Understanding SD's impact on cellular processes is crucial for comprehending these disorders.

Purpose of the Study:

To investigate the temporal kinetics of cortical gene expression changes induced by repetitive spreading depression (SD) in a rat model.
To identify specific genes and pathways affected by SD over an extended period.

Main Methods:

Repetitive SD was induced in rat cerebral cortex using topical 3 M KCl application.
Cortical gene expression was analyzed using Affymetrix RAE230A arrays for up to 30 days.
Microarray results were validated using real-time PCR and immunohistochemistry.

Main Results:

A rapid expression of immediate early genes, inflammation, metabolism, stress, DNA repair, ion transport, and growth/differentiation genes was observed.
Stress-response genes persisted for over 24 hours, with genes related to cell membrane, adhesion, and cytoskeleton being prominent.
A subset of genes remained affected even 30 days post-SD, indicating long-term molecular alterations.

Conclusions:

Repetitive SD induces a distinct temporal pattern of gene expression changes in the cerebral cortex.
These molecular changes may facilitate tissue remodeling, enhance cortical plasticity, and contribute to neuronal tolerance against subsequent ischemic events.