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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
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Comparison of RNA assay methods used to normalize cDNA for quantitative real-time PCR

Eun Joo Lee1, Thomas D Schmittgen

  • 1College of Pharmacy, Ohio State University, Columbus, OH 43210, USA.

Analytical Biochemistry
|August 26, 2006
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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