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Related Experiment Videos

BCR ubiquitination controls BCR-mediated antigen processing and presentation.

Lisa Drake1, Erica M McGovern-Brindisi, James R Drake

  • 1Albany Medical College, Center for Immunology and Microbial Disease, 47 New Scotland Ave, MC-151, Albany, NY 12208, USA. drakej@mail.amc.edu

Blood
|August 26, 2006
PubMed
Summary
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The B cell receptor (BCR) pathway for antigen processing involves ubiquitination, similar to epidermal growth factor receptor (EGFR) trafficking. Inhibiting proteasomes impacts BCR complex trafficking and antigen presentation.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • BCR-mediated antigen processing requires Ag-BCR complex trafficking to MIICs.
  • The molecular mechanisms of Ag-BCR trafficking to and within MIICs are not fully understood.
  • EGFR trafficking to MVBs is a well-characterized ubiquitin-dependent process.

Purpose of the Study:

  • To investigate the molecular mechanism of Ag-BCR complex trafficking to and within MIICs.
  • To determine if ubiquitination plays a role in Ag-BCR trafficking.
  • To compare Ag-BCR trafficking to EGFR trafficking.

Main Methods:

  • Utilized a characterized antigen-specific model system.
  • Examined ubiquitination of the IgM BCR immunoglobulin heavy chain subunit in normal B cells.

Related Experiment Videos

  • Assessed the effects of acute proteasome inhibition on Ag-BCR complex formation, trafficking, and antigen presentation.

    • Main Results:

    • The immunoglobulin heavy chain subunit of the IgM BCR is ubiquitinated in normal B cells.
    • Acute proteasome inhibition delayed ubiquitinated ligand-BCR complex formation.
    • Proteasome inhibition altered Ag-BCR complex intracellular trafficking and selectively blocked BCR-mediated antigen processing and presentation.

    Conclusions:

    • Ag-BCR complex trafficking to and within MVB-like compartments shares similarities with EGFR trafficking.
    • Ubiquitination is a key mechanism in Ag-BCR trafficking to MIICs.
    • EGFR serves as a paradigm for studying Ag-BCR trafficking to MIICs.